Chemiluminescent Assay Stabilisers



Chemiluminescent Assay Stabilisers



Enzyme-linked immunosorbent assays (ELISAs) are among the most extensively used types of immunoassay for diagnostic tests. ELISA detection is based on either colorimetric, fluorescence or chemiluminescence visualisation chemistry. However, the sensitivity of conventional colorimetric detection methods can be ineffective when detecting the presence of analytes in very low abundance (pico to femtogram levels).   Chemiluminescent detection represents a versatile, ultrasensitive tool with a wide range of applications in biotechnology. It is a commonly used immunoassay detection reactions for enzyme or a nanoparticle label. In recent years, chemiluminescent immunoassays have become very popular in clinical chemistry and environmental analysis, due to its high sensitivity, wide dynamic range and complete automation.   For all their versatility and sensitivity, it is well known that the enzymes and substrates involved in both luminol and ATP chemiluminescent reactions are highly unstable in certain environments. The drying process involved in manufacturing an assay or biosensor can be detrimental to maintain good enzyme and substrate stability. Furthermore, storage of these chemiluminescent assays can be a challenge at higher temperatures for prolonged periods.   At Polygenyn Ltd. we have many years expertise in the stabilisation of these assays. We can provide formulations for you to troubleshoot your stability issues in house (see below). We also provide consultancy to help you solve your stability issues. PG03: Protects all the labile components used to make up enzymatic ATP based diagnostic tests against degradation during the drying process and dramatically extends shelf life at elevated temperatures. All of the above stabiliser systems are compatible with most diagnostic assays.